What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Is there Researchers who are already working on anti-viral surfaces. For ex. anti-viral COVID-19 surfaces ? ?

It would be interesting to know, how the scientific community is contributing to the COVID-19 crisis. How chemical/mechanical/material/metallurgy concepts are being used to produce anti-COVID-19...

16 May 2020 5,141 3 View

Data structure and missing values in random forest model?

Hi there, I have over 1 million records, with a categorical response variable and a mix of categorical and continuous predictors. There are several missing values in the dataset, this is...

02 September 2019 494 4 View

Tools/techniques to describe the distribution of cells in a raster, across a study region?

Im trying to analyze and extract patterns from several raster layers, something like what is done for non-raster features. Im interested in learning techniques that describe the distribution of...

02 July 2019 2,333 5 View

Can anyone suggest me how to plot fractional order time delay system in matlab?

I am searching a matlab code for fractional time delay system(continuous case). Still now, i don't got any idea for the above. I need a good suggestion from researcher. Help anyone!!!

06 March 2019 6,097 3 View

Selecting a DC Voltage for EIS Measurements: Can someone help me with this ?

Am confused in deciding the DC Voltage to select for EIS measurement of my ss316L (Coated and Surface modified). Just wanted to crosscheck before proceeding forward. I found this article bit...

12 December 2017 4,913 4 View

How to amplify less copy number gene in Realtime PCR?

I am optimizing real time primers for 10 genes in normal pcr along with GAPDH. Two genes got amplification (GAPDH too) and remaining dosen't. i was using diluted cDNA at the rate of 1:10 as...

25 November 2017 8,132 2 View

Why the RNA got degraded during purification ?

29 March 2017 1,127 2 View

Is it possible to amplify a single copy of dna template (practically) efficiently by using PCR ?

14 March 2017 3,999 11 View

Sleep and Vigilance - would you like to submit your next paper to our new journal?

11 December 2016 1,598 0 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View