I want to amplify P1 baculovirus in suspension cultures, but I do not know the details. Could you please give me a protocol or some detailed suggestions?
How many cells should I seed at the beginning? When should I collect the new-made baculovirus, considering the amount and viability of the new-made baculovirus?
Thank you.
Life Technologies have a very good protocol here:
https://tools.lifetechnologies.com/content/sfs/manuals/baculodirect_man.pdf
Vira Bitko ,Thank you very much!
I read the protocal, and find that cells used to generate a P2 stock are seeded a six-well tissue culture plates, not seeded in a flask. I want to amplify P1 baculovirus in suspention cultures, because someone told me it is better to use suspention cultures, What is your suggestion? Tnank you.
You can do that, just follow the protocol in terms of if you scale up number of cells, you will need more virus from P1 stock (use same MOI, but overall your amount of the virus needed will be higher).
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