i work on Halobacterium salinarum an archaebacteria.
if you know the sequence of your gene so it is not a problem just design a specific primers for your gene and apply them for cDNA formed by random primer
i would like to amplify arcA, a part of the arcRACB gene cluster in Halobacterium salinarum after reverse transcriptase PCR. However i am pretty much worried the product size of arcA in the...
11 December 2012 2,740 4 View
I've never done RT PCR assays before. I'm an independent researcher and am worried that multiplex assays are more expensive than singleplex. However, I'm not sure singleplex assays will give...
11 December 2012 6,761 7 View
Codon usage of a virus must coevolve with its host for the virus to use the host machinery more efficiently and therefore replicate faster. When the codon usage of a virus becomes too deviated...
11 December 2012 2,767 14 View
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I exposed my bacteria to different factors which may affect the expression of a gene. However it has affected the growth of one of my bacteria exposed in a certain stimuli. I can't compare CT...
10 November 2012 10,082 7 View
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10 November 2012 5,369 2 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View