11 November 2012 1 7K Report

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More mark anthony Carreon's questions See All
Are there special considerations in primer design of a gene within a gene cluster?

i would like to amplify arcA, a part of the arcRACB gene cluster in Halobacterium salinarum after reverse transcriptase PCR. However i am pretty much worried the product size of arcA in the...

11 December 2012 2,740 4 View

How accurate are singleplex reactions compared to multiplex reactions in real time PCR assays?

I've never done RT PCR assays before. I'm an independent researcher and am worried that multiplex assays are more expensive than singleplex. However, I'm not sure singleplex assays will give...

11 December 2012 6,761 7 View

Is it normal, for a virus and its host, to have too different codon usages?

Codon usage of a virus must coevolve with its host for the virus to use the host machinery more efficiently and therefore replicate faster. When the codon usage of a virus becomes too deviated...

11 December 2012 2,767 14 View

After isolation of microorganisms, do you usually screen the 16s rna first with restriction fragments before sending them for sequencing?

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10 November 2012 527 4 View

My incubator doesn't have light for the culture of phototrophic microorganisms. Any suggestions for low cost remedies?

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10 November 2012 9,324 6 View

I'm relatively new in rt PCR analysis. How do we normalize in rt PCR using housekeeping genes?

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10 November 2012 6,154 10 View

Is it possible to compare gene expression levels between two bacterial culture (with uneven population)?

I exposed my bacteria to different factors which may affect the expression of a gene. However it has affected the growth of one of my bacteria exposed in a certain stimuli. I can't compare CT...

10 November 2012 10,082 7 View

In comparing mean delta Ct values between treated and untreated samples in RT PCR, what are the statistical tools that you mostly use?

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10 November 2012 5,700 11 View

How can you account for the relative instability of mRNA compared to DNA?

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10 November 2012 5,369 2 View

Can we use the same primer for 16s among different strains of bacteria?

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10 November 2012 1,303 12 View

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