mark anthony Carreon

23 Questions 35 Answers 0 Followers

Questions related from mark anthony Carreon

Are there special considerations in primer design of a gene within a gene cluster?

i would like to amplify arcA, a part of the arcRACB gene cluster in Halobacterium salinarum after reverse transcriptase PCR. However i am pretty much worried the product size of arcA in the...

12 December 2012 2,707 4 View

How accurate are singleplex reactions compared to multiplex reactions in real time PCR assays?

I've never done RT PCR assays before. I'm an independent researcher and am worried that multiplex assays are more expensive than singleplex. However, I'm not sure singleplex assays will give...

12 December 2012 6,729 7 View

Is it normal, for a virus and its host, to have too different codon usages?

Codon usage of a virus must coevolve with its host for the virus to use the host machinery more efficiently and therefore replicate faster. When the codon usage of a virus becomes too deviated...

12 December 2012 2,723 14 View

Will primers work on genes located in the plasmid?

11 November 2012 1,947 4 View

After isolation of microorganisms, do you usually screen the 16s rna first with restriction fragments before sending them for sequencing?

11 November 2012 495 4 View

How can you account for the relative instability of mRNA compared to DNA?

11 November 2012 5,330 2 View

Can we use the same primer for 16s among different strains of bacteria?

11 November 2012 1,259 12 View

Is it possible to compare gene expression levels between two bacterial culture (with uneven population)?

I exposed my bacteria to different factors which may affect the expression of a gene. However it has affected the growth of one of my bacteria exposed in a certain stimuli. I can't compare CT...

11 November 2012 10,052 7 View

Is the process of cDNA purification prior to RT PCR analysis always advisable?

I am worried about the low specificity of SYBR.

11 November 2012 7,250 11 View

In comparing mean delta Ct values between treated and untreated samples in RT PCR, what are the statistical tools that you mostly use?

11 November 2012 5,661 11 View

I'm relatively new in rt PCR analysis. How do we normalize in rt PCR using housekeeping genes?

11 November 2012 6,117 10 View

Is there any protocol for the estimation of carbon content of the different parts of bamboo tree?

11 November 2012 7,295 1 View

My incubator doesn't have light for the culture of phototrophic microorganisms. Any suggestions for low cost remedies?

11 November 2012 9,281 6 View

Why is it that 16s rRNA is being used in inferring relationships among organisms?

11 November 2012 1,342 13 View

Can I use 16sRNA as an internal control gene in RT PCR analysis?

10 October 2012 2,181 14 View

How do we measure gene expression levels of genes in bacteria using RT PCR?

i work on Halobacterium salinarum an archaebacteria.

10 October 2012 8,311 1 View

In a medium with bacteria, how do you quantify gene expression at the same time as quantifying degradative products?

10 October 2012 1,125 2 View

In identification of bacteria, are restriction digests reliable indicators of bacterial identity or do we still need sequencing?

10 October 2012 8,330 13 View

How can you quantify gene expression for a arginine deiminase in archae gene while quantifying the amount of arginine in the medium?

10 October 2012 1,168 3 View

Which is more reliable in gene expression analysis, northern blot or rt pcr?

10 October 2012 5,383 15 View

Im relatively new in rt pcr analysis. Any tips for beginners? What are some of the most common problems encountered?

10 October 2012 6,776 4 View

What is the best statistical tool for this type of data?

I am relatively new to statistical methods.

10 October 2012 4,850 7 View

What are some of the most commonly used sampling methods in water systems for analysis?

10 October 2012 5,812 9 View