We intend to culture individual islet cell to map out the cell lineage.
I recently isolated mice cardiomyocytes and found some are not individual cells. I tried to stain the cells with Wheat Germ Agulutinin, it did not work. Does anyone have recommendations on the...
20 June 2024 3,225 2 View
Nutrient recovery is becoming the order of the day as purchasing NPK has become more costly lately. In the advent of food security issues, one is tempted to get cheaper means to produce nutrients...
15 May 2024 767 1 View
I cultivate CAF from the specimen of the pancreatic cancer patient who had pancreaticoduodenectomy (PD). Other than the medium, FBS, antibiotics, is EGFR needed for the culture?
07 April 2024 8,937 0 View
Which protocols are the best and least cost-effective in the analysis of surface water, biota, and sediment samples? Kindly just list the steps for me and if possible add references to it.
19 January 2024 8,847 2 View
I am trying transfection of SiRNA of COPB2 to pancreatic cell lines in 96 well. Bxpc-3 is difficult to transfect SiRNA following the manufacture's protocol (lipofectamine 3000). So, I have...
26 December 2023 4,059 1 View
If you go through scientific publications, you freely realize that descriptive statistics for categorical variables are presented as frequency and proportion but in two different ways. A)...
23 October 2023 9,651 3 View
I need a table with the standard limits of heavy metals in agricultural soil
17 September 2023 5,989 3 View
I would like to know candidate materials for the fusion reactor and the current research focus on such materials.
22 July 2023 8,172 2 View
Literature ideas on: - Lyrical advocacy - Aesthetics and performance - Historical differences - Legacy All ideas/suggestions/contributions are welcome. Thanks.
20 July 2023 5,106 3 View
I have several frozen white blood cell samples from human cord blood that I would like to isolate neutrophils from whiles keeping the rest of the white blood cell fractions. I know that the Ficoll...
26 April 2023 675 0 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...
10 August 2024 6,493 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View