I am facing difficulty in estimating cell quantification in my scaffold. Usually live/dead assay and MTT is performed to check cell viability. But what if we can extract cells directly from scaffold and measure it using a cell counter.
Dear colleague
Using XTT rather than MTT in which soluble product is obtained would be a better choice when cells are seeded within a scaffold. Nevertheless, if you insist in extracting cells, I cannot guide you with no information about the nature of scaffold.
Hello Alireza,
My scaffold is composed of gelatin and pectin. We are facing difficulty in assessing cell viability using common techniques. That's why I am insisting on extracting cells directly from scaffold. Even in live/dead assay, auto-fluorescence is one of the biggest challenge we are facing.
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