Kamalnayan Tibrewal

5 Questions 4 Answers 0 Followers

Questions related from Kamalnayan Tibrewal

How to carry out selective hydrogenation of vanillin to vanillyl alcohol using Ni-Cu catalyst?

I have experimented with various methodologies, but I am getting p-cresol in end instead of vanillyl alcohol. Can anyone suggest any suitable parameters?

27 March 2023 6,200 2 View

What is the inference behind checking cell viability on a scaffold till 21 days only?

In most of the research papers reported till now on scaffold have shown cell bio-compatibility check using live/dead assay in frequent intervals till 21 days. Even when we are going for...

17 February 2023 9,556 0 View

How do we extract cells from scaffold for estimating its quantification?

I am facing difficulty in estimating cell quantification in my scaffold. Usually live/dead assay and MTT is performed to check cell viability. But what if we can extract cells directly from...

12 January 2023 655 3 View

How to do Live/Dead assay of Cell-embedded Gelatin-Alginate scaffold using confocal microscopy?

I am facing problem with calcein staining as only dead cells are visible during confocal. I could clearly see cell differentiation in phase contrast microscope but at time of LD assay not a single...

10 December 2022 1,821 0 View

For edible scaffold development, I am facing issues while crosslinking gelatin with citric acid. So how can I optimize that?

Along with gelatin, I am also using sodium alginate in the bioink. So alginate is getting crosslinked with CaCl2, but the construct is getting degraded due to un-crosslinked gelatin. I tried to...

15 July 2022 9,290 3 View