How can we quantitatively determine Glacial acetic acid in formulation using HPLC when sodium acetate is present in the same formulation using HPLC?
Thanks for your answer we have 3.2mg of GAA and 17 mg of Sodium acetate per 5mL in formulation, when we went for titration method it doesn't matched with standard using phenaphthalaien as indicator. Can you suggest me a suitable method.
Remember: "Glacial" acetic acid is the name for anhydrous, i.e., water-free acetic acid, therefore it doesn't exist as such in solution. It's a weak acid, so - in solution - it'll be in equilibrium with acetate ion, and the acetate will be indistinguishable from that from the sodium acetate that was added. As suggested by Mark Krause, titration is an option, but will at best give an indication of the total acetate.
Dear Sir
Thanks for you answer, in that case, can you suggest me "How should I forward to estimate total acetate content in above prescribed dosage (3.2 mg GAA & 17 mg Sodium acetate?
You can use kits, e.g.,
http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Bulletin/1/mak086bul.pdf
http://www.biovision.com/manuals/K658.pdf?osCsid=v4lshs9p99k9drm93ki1e6sud6
Or classical methods such as these:
https://www.dartmouth.edu/~chemlab/chem3-5/acid1/full_text/chemistry.html
http://www.kbcc.cuny.edu/academicDepartments/PHYSCI/PL/chm11/Documents/Vinegar.pdf
Dear Sir
Thanks for your mail
Can I apply the final method (from above link) for quantitative determination of GAA and Sodium acetate trihydrate (3.2 mg and 17 mg respectively) in oral liquid dosage form?
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