I have read several articles mentioning the preparation of the drug disulfiram (DSF) in olive oil for oral gavage of rodents. However, in those papers, there is no description of how this is achieved. For one of our projects, we plan to use this approach, and we tried to resuspend DSF directly in olive oil at room temperature at a concentration of 12.5mg/ml, but this is not working properly. Heating helps but we may have a risk to alter the drug. Has anybody experience with DSF/Olive oil preparation? Many thanks in advance, Olivier