What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How much cell pressure and back pressure should I apply to start for triaxial test for different types of soil?

We have recently purchased a triaxial test instrument (MATEST) for testing different types of soil. After installing software, we are facing a issue of saturation where the samples does not...

23 February 2021 7,194 4 View

How can I increase cDNA concentartion obtained from viral RNA?

I need good quality and quantity of viral cDNA for NGS analysis. Please suggest how can I increase amount of cDNA obtained from viral RNA.

02 February 2021 8,379 3 View

Spatial thinking and geographical reasoning: what are their conceptual differences and interrelations in teaching geography?

I am conducting a state-of-the-art analysis of these concepts. If you have contributions, please, send me.

17 January 2021 1,123 3 View

Why there is need to calculate the chlorophyll content and chlorophyll index. What protocol should I used for calculating chlorphyll in mustard?

I want to know the best and effective method/ protocol to calculate the chlorophyll from mustard plants. Please suggest some relevant and the methods or protocols from which I can easily calculate...

05 January 2021 9,882 3 View

Are there any way to make the comparison between different analysis of reliability possible?

10 December 2020 7,922 4 View

How much Tensile strength and hardness can be acheived in Aluminium 7050 MMC with B4C reinforcement?

I have conducted research on characterisation of Al7050 + B4C by electromagnetic stir casting. The alloy employed in the process is casted alloy and the Composite too is casted. How much strength...

16 November 2020 9,226 3 View

Can u suggest how to identify the matrix material and reinforcement particle distribution in the MMC of scanning electron miroscopy (SEM) images?

How to identify the reinforcement particle behavior in SEM images

09 November 2020 8,664 3 View

Hi; I need some MRI or CT scan pictures of the human brain. Can anyone suggest me any good website for finding these files?

need a human brain MRI/CT scan image dataset for research purposes.

08 November 2020 2,145 2 View

Nano Ice films sublimate due to low dose beam and annealing under vacuum? which one is more likely dominant?

An ice film sublimates under a severe vacuum pressure and a low does of electron beam. when the sample is in-situ annealed the sublimation rate significantly induced. In an isobaric process,...

06 November 2020 7,576 2 View

Why this SSR primer show band like that ?

Each time this SSR primer show band like that other show one or two band . Is it polymorphic ?

11 January 2021 2,221 5 View

What is the best quality DNA for SSR fingerprinting?

Nanodrop result of my DNA samples extracted from fresh finger millet leaves showed 260/280 ratio ranged from 1.7-2.2 whereas 260/230 ratio ranged from 1.1-2.2. How can I assess the quality of such...

30 November 2020 8,689 6 View

Weak SSR peak on capillary electrophoresis trace - is it a heterozygote?

Looking at the attached image, the top two samples show a weak peak at 212 compared to the very strong 200 peak. The bottom samples is homozygous for the 212 peak. Since the samples look a bit...

27 October 2020 2,353 3 View

What tool can I use for creating a Quantative Trait Loci map from SSR data?

I have SSR data for some individual of three hybride How I construct QTL mapping of yield according to these data ?

02 October 2020 502 5 View

How can I differentiate the true allele from stutters and PCR artifacts in microsatellite genotyping?

Most of my microsatellite markers are dinucleotides, therefore they show stuttering. In regular stuttering is easy to identify the allele, which is the highest peak on the right, after the shorter...

26 September 2020 1,486 5 View

How to calculate marker-trait association?

there are many method to calculate marker-trait association, namely stepwise regression, GLM, etc. Before running those methods, how we define the X (independent variables) and Y (respon...

14 September 2020 6,689 2 View

No amplification of SSR markers on Pecan nuts project?

Hi all, I am doing an SSR project on Pecan nuts and I am struggling to get amplification on my PCR. Here is a summary of what I have done so far 1. DNA extraction was a modified CTAB protocol...

19 April 2020 8,678 2 View

Why are microsatellite stutter peaks changing on repeated fragment analysis runs of same PCR product?

I've run a couple hundred samples using a microsatellite panel, and recently some of the runs show very exaggerated stutter peaks smaller than the true allele size. To troubleshoot the issue,...

01 April 2020 4,501 7 View

Cattle microsatellite data? Who is interesting to send me his data?

Dear I'm in process to do a big data microsatellites analysis about all cattle breeds around a world. I'm very gratitude for all researchers accept help me in this project; sure any researcher...

29 March 2020 6,549 3 View

Why all trangenic mice are negative on gel while both their parents are positive?

We created parent mice with long trinucleotide repeats. We did genotyping on offspring mice and it worked well. But the most recent generation showed no band at all. We checked the genotype of...

27 March 2020 8,776 6 View