How do I tell if the sample is heterozygous or homozygous for a microsatellite marker from a chromatogram?
I got comment on my FTIR data figure from a reviewer. The reviewer said "FTIR data in Figure should be repeated. there is no bassline." I made Y off set comparison graph of FTIR on OriginLab. Can...
03 August 2024 6,070 3 View
I am looking for journals that admit the publication of results from applying the IUCN Standard for designing and monitoring Nature-based solutions. Many thanks for considering my request.
23 July 2024 6,444 2 View
Hello, I want to load Remedesivir (RNA polymerase inhibitor) into mesoporous nanoparticles (MSNs). The issue is drug is temperature and pH sensitive and it degrades quickly. I need advice on its...
01 July 2024 889 3 View
Hello I have prepared metal nanoparticles and did zeta potential. Normally there is a single peak in sample but now there are 3 peaks. I prepared sample for analysis first I prepared 10 mg/mL...
30 June 2024 1,063 4 View
When I examined the GIXRD data obtained recently, I observed that the sample treated with O2 plasma on the Cu surface deposited on Si chips showed increased intensity compared to the sample...
19 June 2024 6,900 5 View
I am working on an acetyltransferase that is highly unstable. Its pI is 6.65, and its molecular weight is around 18 kDa. The protein elutes at 1M imidazole and begins to precipitate immediately...
19 June 2024 2,590 0 View
I conducted GIXRD measurements after performing O2 plasma treatment on a Cu surface. It was observed that the baseline is significantly high at low angles (10-30 degrees). What could this...
18 June 2024 2,996 3 View
I attempted to model a temporary 'lane closure' due to an accident or work zone. Initially, I successfully closed Lane 1 on Link 1, and it worked as expected. However, when I tried to close Lane 1...
17 June 2024 2,070 0 View
Hello guys. I have faced the problem of being in need of carboxyl-methylated amino acids. Amino acids I need are not available in the market so I need to synthesize them by myself. So thus I'm...
16 June 2024 9,920 3 View
I found a variant segregating with a genetic condition, this variant is listed in ClinVar (with no clinical significance), gnomAD, etc. However, I could not find any publication reporting the...
10 June 2024 7,606 3 View
I’m currently working on polyglutamine disease by Sanger sequencing to count the CAG repeats. However, since my targeted genes involve CAT or CAA interruption in the CAG repeats, their...
01 July 2024 8,328 5 View
Good afternoon. Please tell me, are there molecular markers of zeina genes for the purpose of genotyping lines and varieties? There are Asid-PAGE and SDS-PAGE methods for studying the...
25 May 2024 6,960 1 View
For phylogenetic analysis using SSRs, normally we don't use just one SSR, but a set of multiple SSRs to ensure accuracy. So how can we input these data into NTSYS? I know input for one SSR is as...
13 May 2024 1,867 1 View
Hello, I am planning to conduct linkage and parentage analysis using microsatellite markers (SSR). The scoring process has been completed with output in allele size (bp) format. The analysis is...
12 May 2024 5,329 0 View
Hello everyone, I'm currently conducting genetic analyses using SSR markers, and I'm looking for software recommendations that support GLM (General Linear Model) and MLM (Mixed Linear Model)...
20 March 2024 5,749 1 View
Hi, I have a doubt. I am working with microsatellites developed for a Polyploid plant species (Schoenoplectus americanus) which I have transferred to the species of the same genus (Schoenoplectus...
11 March 2024 5,205 4 View
I want to study the genetic diversity and population structure of my samples using SSR markers. The PCR products of my samples show many bands of different sizes. and i am confused about how to...
06 March 2024 3,177 1 View
In the SSR method, I used the same pair of primers (forward and reverse) in two different individuals, but I observed different band patterns in PCR.
05 February 2024 3,438 2 View
Genetic Engineering. Assuming that there were no errors during the process.
03 February 2024 2,832 0 View
Dear All I am trying to amplify the SSR markers on genomic DNA of date palm but it always gives smeared, diffused and fuzzy bands in gel electrophoresis. I have standerdized them as much as the...
01 February 2024 6,534 3 View