Hello,

I am planning to conduct linkage and parentage analysis using microsatellite markers (SSR). The scoring process has been completed with output in allele size (bp) format. The analysis is planned to be conducted using the rqtl package in RStudio, so the allele data needs to be converted into genotype formats like A, H, B, etc. (by comparing them with the allele sizes of the parents). Is there any guidance or reference for performing this conversion because I am a bit confused about it, for example:

  • If parent A has alleles of 100 - 120 bp, while parent B has 100 - 100 bp alleles, should progeny with allele sizes of 100 - 120 be converted to A (identical to parent A), or H (combination of both parents)?
  • If an allele appears that is not present in either parent, how should it be converted?
  • Please provide information if there are any software/packages/web tools that can perform this conversion automatically.

    Best Regards

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