Dear All
I am trying to amplify the SSR markers on genomic DNA of date palm but it always gives smeared, diffused and fuzzy bands in gel electrophoresis. I have standerdized them as much as the posible ways but still I am getting same result. Why such result is happening> I am attaching gel image herewith for reference. Please suggest.
Regards
Chet Ram, Ph.D.
Chet Ram Sir, possible reasons could be degradation of DNA or too much concentration of template DNA in PCR or Primer concentration not optimal or primers degraded or due to annealing temperature of primers (try optimising the annealing temperatures using gradient pcr), High number of pcr cycles .
Chet Ram Look at the answer of @Ľuboš Majeský to the similar question: https://www.researchgate.net/post/Why_do_I_get_smeared_bands_on_PAGE_of_SSR_markers
Hello
It is suggested:
Re-optimization of primers annealing temperature
Using standard concentration of forward and reverse primers
Use hot-start PCR
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