I induced diabetes with STZ (40 mg/kg) after 4 weeks of high fat diet in Wistar rats. The rats are showing inconsistent blood glucose level. Why?

I induced diabetes with STZ (40 mg/kg) after 4 weeks of high fat diet in Wistar rats. After 72 hours, i checked the FBG of all the rats and majority of the rats had FBG above 350 mg/dl. After a...

24 September 2020 5,975 24 View

Global warming challenges, what's the way to go?

New discussion While some still consider climate change majorly caused by GHGs as a hoax, it is impossible to rule out a striking correlation between the increasing concentration of atmospheric...

28 October 2019 9,301 24 View

Is it possible to connect dc motor directly to dc bus?

I want to know if is possible to connect dc motor directly to dc bus. I don't know if is possible to eliminate the converter and make use of DC Motor directly.

26 March 2019 3,602 5 View

How do I go about Maxi Prep Preparation?

12 June 2017 3,060 3 View

Is it possible to hybridize two classifers ANN and SVM for prediction?

if possible. how will i go about it, if ANN is working already?

13 December 2016 9,198 6 View

Sample size

10 November 2016 4,224 5 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

What is the right way to conjugate two acids?

How to conjugate two acids with amine linker?

18 February 2021 4,083 2 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View