How do you prepare trolox standard curve for ABTS assay ?

Opeyemi Deji-Oloruntoba @Opeyemi-Deji-Oloruntoba

03 July 2023 0 4K Report

I have tried a range of concentration, but I guess there is something I am not getting. Also, The literatures I am following on the dilution of my ABTS working solution are varying, Please i need a clear protocol. Thanks

Badges
Science topic

More Opeyemi Deji-Oloruntoba's questions See All

Which software tools are best for enhancing diagnostic accuracy in chest X-ray imaging using image reconstruction and neural networks?

I am reaching out to seek your valuable advice and recommendations regarding the best software tools to use for this research. Specifically, I am looking for software with a user-friendly...

22 July 2024 3,794 1 View

What are the best research techniques for a cross examination study targeting engineers, architects, and quantity surveyors?

25 April 2024 9,762 2 View

Any recommendations on methodologies for a lab-based assessment of the effects of elevated CO2 on soil heavy metal bioavailabilty?

Existing literature use the FACE systems, SACC, OTCs, and greenhouses. These are large-scale and also study plant responses. I intend to work with soil samples alone, and I can't find suitable...

10 March 2024 9,124 2 View

Preparation of plant extracts for antimicrobial analysis?

antimicrobial

11 November 2023 6,271 4 View

Which schemes would perform better than improved Feige–Fiat–Shamir (IFFS) for modeling a secure IoT-integrated WBAN framework for e-healthcare?

Which authentication schemes would perform better than improved Feige–Fiat–Shamir (IFFS) for modeling a secure IoT-integrated WBAN framework for e-healthcare?

01 August 2023 675 4 View

Can i get residential estates in Southwest Nigeria where Compressed Stabilized Soil blocks Or Interlocking Stabilized Soil Blocks (ISSBS) are used?

Compressed Stabilized Soil Block and Interlocking Stabilized Soil Blocks are unfired earth masonry units used as walling material. They are machined pressed and come with different names. I need...

07 May 2023 9,373 1 View

Copper-free click chemistry conjugation of DBCO modified quantum dots and azide modified olighonucleotides?

I have been trying to conjugate DBCO (Dibenzocyclooctyne) modified quantum dots to azide modified oligonucleotides. I havent been able to get this to work well. Can anyone recommend a protocol for...

05 May 2023 1,835 3 View

What HPLC program works best for separating unbound azides in a SPAAC reaction ?

I conjugated DBCO modified quantum to azide modified oligonucleotides using SPAAC click chemistry, I have been trying to use HPLC SEC to separate unbound azides and characterize the conjugation....

27 April 2023 8,630 2 View

Bee Venom and Cancer: Can Bees Offer a Cure?

I'm a medical writer and editor interested in learning more about the potential of bee venom as a cancer treatment. I've read that bee venom contains a variety of proteins and enzymes, including...

26 April 2023 7,894 2 View

What is the software for identifying compounds from LCMS analysis?

I used AMDIS to identify peaks from Agilent GCMS (.D file). I was under the impression that the said AMDIS would also be able to identify peaks from LCMS, however, I have not been able to open the...

05 January 2023 2,165 4 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,620 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View

What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

12 July 2024 6,313 0 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,830 1 View

How to assess the viability and manage debris in fish primary cell cultures?

Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...

10 July 2024 4,918 2 View

What factors could be contributing on low tumor take in female Nude mice?

Hello everyone, I'm working with a PA-1 ovarian cancer cell line xenograft model in female nude mice. My cells are at 372 passages, the cell repository bank themselves gave at 352th passage....

05 July 2024 5,860 1 View