How do I quantify plasmid using a DNA ladder in agarose?

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Selectively visualize his-tag fusion proteins

I need to know if there's a way I can specifically quantify (visually) his-tag fusion protein. I have checked for commercial his-tag stain reagents, but I'm looking for state-of-art method(s)....

04 May 2013 8,555 4 View

Can someone help me with DNA polymerase activity analysis?

I've expressed Taq polymerase in E.coli. I have found a few reports for its purification also using sephadex columns. Next, I need to check its efficacy preferably by methods other than PCR. Can...

04 May 2013 971 2 View

How to separate/purify B lymphocytes from blood/spleen?

I am looking for cheaper and still efficient state of art methods. I have checked for FACS and a few commercial kits but they are out of my budget.

02 March 2013 1,868 8 View

What is Pull-through PCR?

In their work (see attachment), Pansri et. al. has used pull-through PCR. I am unable to understand the purpose because I do not know about Pull-through PCR.

01 February 2013 7,201 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View