I am working with e.coli. I need help with interpretation of the results. I have worked with two E.coli strains. I did triplet technical replicates. I need to do statistical T-test.
Below is some of my datta:
Strain 1 0,407 0,544 0,639 Ave.: 0,53
Strain 2 0,384 0,515 0,34 Ave.: 0,413
Negativ control: 0,161 0,181 0,14 Ave.: 0,160667
Can someone help me with this. Please. There are several methods that are available to measure bacterial biofilm adherence. I was told to do a t-test beetween my average negatic control and a strain of E.coli.
PLEASE HELP
Thank you
Hi Maryam, are these results of optical densities measuring at 600 nm?
Have you made calibrations of spectrophotometric readings vs. bacterial cell densities prior to the test? These data are necessary for any further statistical analysis.
I would like to recommend the following article:
Corte, L., Casagrande Pierantoni, D., Tascini, C., Roscini, L., & Cardinali, G. (2019). Biofilm specific activity: a measure to quantify microbial biofilm. Microorganisms, 7(3), 73.
Dear Maryam,
Why don't you simply subtract the mean negative control values from each of the two samples, and then do a T-test between these two means? There are many web sites that will let you do a T-test - but you could download and use JAMOVI too (it is free). My quick test shows that there is no significant different (t-test, p = 0.243).
Andrew
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