I have lung images in which I have used QuPath software to quantify total alveolar space damage. I am attempting to validate the method via linear mean intercept (LMI) measurements, however I am having trouble with the LMI method.
From what I understand I need to use ImageJ to lay a grid over my images and measure the number of intersections the tissue has with the grid (analyze particles), however I am unsure how to properly exclude any regions where there are arteries and airways which would not be considered alveolar space. Is it possible for me to draw a polygon around these regions and exclude them from the quantification? Any help is greatly appreciated!
Unfamiliar with application here, but if you want to exclude regions from particle analysis, apply a mask. Duplicate image, add 1 to every pixel, draw a region, clear it out, turn into binary, and apply as mask (there's a plugin, or just multiply images) before doing particle analysis
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