Matt Jackson

3 Questions 26 Answers 0 Followers

Questions related from Matt Jackson

What is the purpose of Sephadex G-100 separation after PEG precipitation for exosomes/EVs in a publication?

Hello! I am reviewing a procedure for exosome/EV purification from a large-volume blood sample (porcine, terminal draw). In brief, protocol is: 1) Blood collection 2) Two-step spin to get...

27 October 2023 6,872 1 View

Anyone have some advice on labeling glutamic/aspartic acids on enzymes/proteins with amine/cadaverine dyes?

Looking to label a enzyme (at it's -COOH groups) with an dye with an amine or cadaverine group. (I can't use an NHS ester here, two important sites of the enzyme would be compromised). There are...

21 May 2019 6,160 2 View

SYBR Gold staining on a precast gel containing ethidium bromide?

I'm working with some precast BioRad gels (1% agarose, up to 96 well format) and want to boost sensitivity for single stranded DNA. Looks like BioRad has been discontinuing products, and the only...

29 January 2019 779 0 View