How do I prepare X-gal plates for transformation purpose ?

02 February 2018 1 5K Report

Please i need somebody to link me to a protocol or help me with how to prepare X-gal plate for selecting my cloned gene .My kit contain 1ML Ampicilin(100g/ml conc), 1ml X-gal, conc(20g/ml and 1ml 1 PTG with conc.500mM . From this concentration how do I prepare my X-gal plates to use for the selection.Thanks

Julian Alexander Zagalak

Hi Suleiman,

Im pretty much following the standard protocol, an example of which can be found here on the goldbio.com homepage.

https://www.goldbio.com/documents/1031/Blue-White+Screening+Procedure2.pdf

Good luck,

Julian

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