Suleiman Allison

5 Questions 3 Answers 0 Followers

Questions related from Suleiman Allison

Can anybody help me with the protocol on how to detect protease activity in a gel containing Casein substrate?

I have just cloned and got a soluble expression(50%) of a thermophilic serine protease and did the heat treatment for purification at 70 and 80 degree with over 90% purity, and also have a...

03 March 2019 5,571 3 View

What are best purification buffers and their conc. that I can use to purify a 6xHis- tagged recombinant serine protease using Ni-NTA resin ?

I have just cloned and expressed a thermophilic alkaline serine protease in E.coli BL21(D3). The protease was expressed as a soluble protein (about 60%) with about 30kDa Mol. wt. (confirmed by...

12 December 2018 7,431 8 View

How do I prepare X-gal plates for transformation purpose ?

Please i need somebody to link me to a protocol or help me with how to prepare X-gal plate for selecting my cloned gene .My kit contain 1ML Ampicilin(100g/ml conc), 1ml X-gal, conc(20g/ml and...

02 February 2018 5,434 1 View

Can I design primers to amplify gene for thermophilic enzyme from the sequences of non-thermophilic enzyme?

I want to amplify a gene for thermophilic protease from highly thermophilic bacteria that grows well at 70 degrees and above and produces protease enzyme at that temperature even on plate. If I...

10 October 2017 9,269 0 View

What is best temperature for the incubation of thermophilic bacteria on liquid broth for the purpose of DNA extraction?

I incubated my organism (thermophillic bacteria) on LB broth at 37 and 40 degrees centigrade for the purpose of extracting DNA from the organism but did not show any growth after 22hrs of...

10 October 2017 3,279 12 View