I want to amplify a gene for thermophilic protease from highly thermophilic bacteria that grows well at 70 degrees and above and produces protease enzyme at that temperature even on plate. If I want to design primers to amplify my gene of interest for thermophilic protease, is it necessary to design the primers from the sequences of thermophilic protease or I can use any protease either thermophilic or not for the primer design.