I am working with fresh crayfish gill samples from the field and would like to run PCR to look for mitochondrial DNA gene 16s. I have to lyophilize the DNA without an expensive machine- just want a simple method that works correctly.
Hi Maria,
Simple way is to precipitate your DNA by ethanol. Many protocols are available and only need centrifugations that you use during your extractions. So, you can stop your extraction before the last step: dissolve the pellet in the buffer.
Best regards
If you already have the DNA sample you might consider speed-vacuum drying or evaporating the buffer (in room temperature or in a heated oven) to dry the DNA.
If you do not have a speed-vacuum centrifuge or dying put it at 37°C overnight to dry it out
You can spot it on filter paper. It will air-dry in a few minutes. Later, it's recoverable by adding a drop of water. It works for PCR or plasmid transformation.
Hi, in my work i used a simple device for make lyophilization. Regards.
Thesis Aplicación de la liofilización para la obtención de andamios de PLLA.
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