I have a 10mg Aprotinin vial (sigma A1153), I need to prepare a stock of 4Units/mL from this vial. Can anyone tell me the calculations or how should I prepare this stock solution?
You can dissolve the 10 mg of Aprotinin in 2ml of sterile distilled water. The data sheet recommends solubility of 5mg/mL in H2O. When you do that as per the data sheet you will get the Aprotinin between 3-8U/mg so roughly 25U/mL (assuming 5U/mg) when 10mgs dissolved in 2mL. You can dilute this Aprotinin stock 1:5 to get 1mg/mL or roughly 5U/mL working stock.