I am currently trying to optimise gel formation using single stranded DNA synthesised and dissolved in range free water. I am having a problem with the formation. Basically when I add my single stranded DNA together to anneal and form the individual pairing scaffolds (Y-scaffold with L-linker), I can visibly see some precipitation after heating at 95 oC. I have not tried any salt concentration for this run. With another run I tried 100mM NaCL with 20mM tris added but could not form the gel either. Basically they should anneal together..Can anyone offer any idea for my optimisation?
Actually the formation of hydro gels require the cross linking between the molecules.And in case of annealing only one strand is annealing to another strand and cross link is absent.
Modify the DNA to act as a cross-linker and add cross-linking agent to from DNA hydrogel.
U can refer attached publication to get something relating to formation of Hydrogel.
Article Responsive DNA-Based Hydrogels and Their Applications
Thank you so much for the input. I optimised my buffer conditions and it worked!
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