Umesh Goyal

2 Questions 3 Answers 0 Followers

Questions related from Umesh Goyal

What will be the good protocol to knockdown specific gene using siRNA and followed with 48hr of serum starvation treatment?

I am working on human MSCs under serum deprived condition, right know i want to do siRNA transfection followed with 36-48hr of serum straved treatment. What would be a proper protocol for getting...

11 November 2018 7,679 0 View

With which parameter i should normalise my ELISA readings fro cytokine done with conditioned media collected from different treatment?

I am doing cytokine ELISA using supernatnat collected from conditioned media of different treatment. However in my different treatment cell growth is very different, I mean cells grow very slow...

01 January 1970 2,934 6 View