Hi,

Even I have the same question. I am in need of DNA from Zoanthids. I tried one method of DNA extraction from Sea anemones, from which I was successful in first attempt. You may try that method.

This is for your reference.

Article DNA extraction from sea anemone (Cnidaria: Actiniaria) tissu...

Try the DNA extraction protocol as used by Varela et al. (2009)

http://onlinelibrary.wiley.com/doi/10.1111/j.1755-0998.2009.02674.x/abstract

i followed DNA extraction by phenol chloroform method ....sambrook et.al

hi try this, Samples preserved in ethanol were rehydrated in sterile distilled water for 1 h. About 50 mg of tissue was taken and add 1 ml of guanidine hydrochloride buffer (10 mM Tris HCl, pH 8.0, 0.1 M EDTA, pH 8.0, 6 M guanidine hydrochloride and 0.1 M sodium acetate) and homogenized well,Then the homogenate was transferred to a 1.5 ml microfuge tube allowed to react for 30 min at room temp, then centrifuged at 5000rpm for 5 min at 4°C. Supernatant was transferred to a fresh microfuge tube and add equal amount of 100% ethanol centrifuged at 14,000rpm for 20 min at 4°C, discard the supernatant then add 95% ethanol centrifuged at 14,000rpm for 10 min at 4°C,discard the supernatant then add 70% ethanol centrifuged at 14,000rpm for 10 min at 4°C,discard the supernatant,Then the DNA pellet was dried in a vacuum drier and dissolved in 50 μl sterile distilled water. A portion of this extract was used for

PCR assays.

Thank you Mr. Dinesh. I also will use this protocol. may be of some help. because even these days I am not getting results.

The best DNA isolation kit is Qiagen for Marine Species. You can also DNA isolate from Salt and Phenol chloroform, before that you have to check two important point.

In your case Ethanol preservation will may create some problem, check the quality of ethanol, try to use Analytical grade ethanol, and before store in Ethanol, you kindly check the quality of shrimp specimen, if it is started to degrade or spoiled one you avoid that one for store

Do well, all the best...

Qiagen DNA extraction have been successfully used, which gave a high through-put

You can try CTAB DNA extraction method after rehydration of samples, maybe it would be necessary to reduce time of incubation in CTAB buffer

http://www.mbari.org/phyto-genome/pdfs/CTAB%20DNA%20Extract_GenomeSeq%20quality.pdf

finally i got dna from both qiagen and Manuel method. now i standarized. anyway thanks to all.......

Which manual method is successful for u Purushotaman. When compared to qiagen and manual method which is the better one wrt to down stream application like  PCR, qRT PCR, Sequencing?

Anna Solovyeva the link doesn't work unfortunately.

How do you rehydrate specimens?