How do I get started with CRISPR system in zebrafish?

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Is it recommended to use DNA LoBind tubes for protein extraction - and how, if at all, does it affect retrieval of proteins?

Hi, in our group we are trying to optimize a protein extraction protocol, and I've noticed DNA LoBind tube and DNA LoBind tips are being used. I wanted to know if it is ok to use DNA LoBind for...

30 June 2024 7,751 2 View

Fluorescence spectroscopy: signal from water?

In order to proceed with the quantification of an Azide Alkyne Cycloaddition reaction efficiency by using a fluorogenic probe with Cy5-DBCO, I first need to construct a calibration curve. The...

16 May 2024 6,266 5 View

Can I calculate the high and low spin of the material in Quantum espresso?

I would like to simulate the high and slow spin configuration of a metal oxide. I am using QE for it, but in my research, I found that this kind of calculus is not possible. Or if it is possible,...

15 May 2024 6,497 3 View

Metai ion (zinc2+) docking score calculation?

Is there a way to calculate the docking score of the zinc bound to this amyloid protein https://www.rcsb.org/structure/1ze9 without undergoing the docking procedure which is apparently not...

01 May 2024 8,951 1 View

Is possible to desorbe CO2, N2 or water in ZIF-8 between 30ºC and 60ºC?

Dear all, After checking DSC and TGA analysis for ZIF-8 samples, it was possible to observe an endotermic peak at around 50ºC, using DSC under N2, and a weight loss in TGA at around 38ºC and 125ºC...

04 April 2024 8,460 4 View

¿Qué recursos son necesarios en Servicios Sociales para tratar de eliminar barreras de acceso laboral en personas con discapacidad?

Con este planteamiento se pretende hacer reflexionar a los lectores de los diferentes planes de actuación, medidas y otros tipos de propuestas que pueden ser implantados en los Servicios Sociales...

10 March 2024 1,481 4 View

¿Qué actuaciones faltan para favorecer una garantía de derechos de las personas en situación de dependencia y/o sus cuidadores?

Con este planteamiento se pretende que los lectores tomen conciencia del enorme problema social que supone en la sociedad andaluza que una persona se encuentre en situación de dependencia. De los...

10 March 2024 6,151 3 View

QE with GPU can be installed in a normal CPU system?

I am trying to install QE with GPU. I used this configure instruction: sudo ./configure --with-cuda=/opt/nvidia/hpc_sdk/Linux_x86_64/24.1/cuda/ --with-cuda-runtime=12.02 --with-cuda-cc=61...

26 February 2024 927 2 View

Greetings!, Can anyone recommend a methodology to maintain water stress in maize, at a laboratory level?

I've seen different concepts in various articles, where they mention "maintaining 50% field capacity" or around 30% humidity. But how do I get to that information?

30 January 2024 8,168 2 View

Degassing metallic-Chitosan beads for BET surface analysis, the sample doesnt resist high temperature with vaccum, recomendations?

Hello community, i have trouble degassing Chitosan beads for BET surface analysis, the sample doesnt resist high temperature with vaccum, any recomendations, previous experience analyzing BET on...

16 January 2024 3,430 1 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...

05 August 2024 853 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View