How do I get rat Recombinant nlrp3?

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Jordan R. Yaron

Do you have the rats available? You could enrich PBMCs from the blood and detect it from the lysates, or IP them out to get a "pure" sample. It should be constitutively present.

Divaker Choubey

The sequence of murine and rat NLRP3 is very similar to each other. Therefore, recombinant murine NLRP3 should work in the assays.