How may i find a researcher who is working on Teachers Engagement?

Hi everyone, my current Ph.D Project is on "Teachers Engagement in STEM teaching" Please I like to link up with (find) another or any researcher working on Teachers Engagement. I will be grateful...

14 January 2021 8,170 5 View

What Explained Variance is good to proceed with Predictive Soil Mapping?

I have 1851 soil samples data on pH covering a study area of 7482sq.km in northern Ghana and I am using 52 environmental long-term average variables (Relief, Climate, MODIS Reflectances and...

27 December 2020 2,728 6 View

How did you feel when you received a notification of the acceptance of your first paper?

As a novice in the world of research with the aim to publish in a peer-reviewed high-quality journal, you do all you can to get quality work. Sometimes the feedback from reviewers can discourage...

09 September 2020 147 31 View

Is it possible to get DOI for previous publications without DOIs?

Have previous publications without DOI with certain journals though they now provide DOIs but don't seems to be ready to link up for the provision of DOIs for previous publications. Any means to...

31 August 2020 9,773 5 View

Reference style "Games for Health Journal"?

Does anybody knows if there is a reference style in Mendeley for "Games for Health Journal" ISSN 21617856, 2161783X?

09 July 2020 8,151 1 View

OK to store pelleted transformed E coli at 4C for 2 days?

Immediately after transforming competent cells with a plasmid ordered from Addgene, I began growing a mini prep culture (5ml) overnight. Next morning, I pelleted the cells and put them in the...

01 February 2020 2,085 2 View

What is the primary structure of a typical keynote address usually delivered at a conference?

Keynote address

07 September 2019 567 7 View

Is adult learning really receiving attention and support globally?

We are living in the world of fast-paced changes, and thanks to ICT. It is really easing learning, especially among the the young adults. That notwithstanding, adult learning seem not to really...

01 March 2019 522 13 View

Some one help, How can I induce glioblastomas in rats?

I am researching on a chemotherapeutic agent for gliomas which i intend to deliver using nanotechnology.

15 February 2019 9,578 4 View

Please I need a PhD research topic?

Hi.i hold a Bachelor of science in Geology, a master of science degree in Geology and a master of science degree in GIS and Remote Sensing. Please I am really in need of a research topic for my...

16 December 2018 4,011 17 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View