Remove an author from my article ?

I need to remove an author from my article as it is not correct , how can I do that ?

16 November 2020 1,391 6 View

Do you know researchers and academics from Tianjin who would fill out a questionnaire about the perception of risks associated with water?

Do you know researchers and academics from Tianjin who would fill out a psychometric questionnaire about the perception of risks associated with water quality, or send this questionnaire to their...

01 November 2020 7,009 4 View

Where GIS shapefiles with city boundaries for several German cities can be found?

I am looking for shapefiles of city boundaries for several German cities (possibly including district boundaries within a city). No other spatial information is required. Do you know where I can...

22 March 2020 3,087 5 View

Can anyone recommend a scale for measuring the hierarchical position in an organisation by self-report?

Dear all, Can anyone recommend a scale for measuring the hierarchical position in an organisation by self-report? The scale should measure the hierarchical position regardless of the industry or...

20 February 2019 2,076 5 View

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Ideally involving the need to calculate significant values and take into account a variety of parameters Current plan is phase, amplitude and height imaging on mica, glass, hopg and calibration...

07 January 2019 6,298 5 View

I'm trying to do a S-W normality test for my data in SPSS. I have 35 items and 5 scales. What should I put to the "Dependent List"?

Should I include the 35 items? Or the 5 scales together? Or should I compute a "Total score" scale? I always get different data. Thank you kindly for your help.

29 December 2016 5,596 3 View

How can I unstick differentiated THP-1 cell (after stimulation by PMA) for analysis on flow cytometry ?

What is the best choise for unstick differentiated THP-1 cells? We tried EDTA, but I would like to observe some markers of differentiated by flow cytometry. Unfortunatelly, after EDTA THP1cells...

30 May 2016 8,079 5 View

Hej, anyone knows how to use e-FTIR software to calculate DE of pectin?

I would like to know the DE of extracted citrus pectin using the software Essential-FTIR. Thanks.

01 May 2016 7,095 1 View

How to calculate a dose of an experimental drug based on its LD50?

Hello everyone! I would very much appreciate any help with the following. I have some experimental compounds which have never been tried in animal studies before, and have no close analogues. The...

01 January 1970 210 9 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View