How to perform CV by using potentiostat?

I have deposited a metal oxide thin film of glass plate, A conductive thin film was also deposited on the glass substrate before the deposition of metal oxide film. How can I perform its CV from a...

01 February 2021 7,710 3 View

Is there applied of types of injective modules with GAP programming ?

If you have any paper about types of injective modules and applied with GAP programming, please send me to the Email: [email protected]

28 December 2020 9,521 2 View

Selection of Steel Frame Section in SAP2000?

Hello, I have a technical question about the selection of steel frame sections in the designing of steel frame structure i.e W16x48 etc. The common practice I have seen is the trial and error...

11 November 2020 5,762 4 View

Can anybody help me with a protocol for Sudan Black B staining for paraffin sections?

Hi I'm planning to use SBB staining for some of my paraffin sections. We have seen unstained areas along the tissue sections in H&E stained sections and also while staining for the mucus...

30 October 2020 1,185 3 View

How can diagnosis the histological changes in rat brain treated with drugs?

Histological changes in brain

10 May 2019 1,935 4 View

What is the benefit of using fluorescent dyes?

What are the immunological indicators that can be obtained from the use of Fluorescence dye?

02 May 2019 7,936 11 View

How to distinguish between histological section in the infected kidney and the healthy kidney?

How to distinguish between histological section in the infected kidney and the healthy kidney

01 May 2019 8,110 13 View

How can I achieve scientific and research collaboration through research gate?

I need help. I'm thinking about it

25 December 2018 9,956 3 View

What is the difference between Thomson Reuters and Impact factor?

Hello everyone I am totally confused between the terms of journals have impact factor, journals have Scopus index number and journals have Thomson Reuters index number. Could anyone explain for...

11 March 2018 9,514 10 View

What are the well known methods available to recycle concrete demolition?

Million tons of concrete blocks are now unneeded because of improved security situation . These blocks must be demolished and recycle as aggregate to produce a new concrete . In this ways we...

08 February 2018 317 5 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View