What is the best method of preparing cell supernatant samples for a Luminex assay to detect cytokines ?

I am planning to run a Luminex assay using cell supernatant to assess cytokine secretion by tumor cells. I came across different methods in literature; some suggest seeding in complete media and...

03 February 2021 4,867 3 View

How to make python look at selective history, in RNN model?

My dataset has 3 columns - a,b,c. Using b (and its history), I wish to predict c. Using list function and converting to array, I can tell python to look at last 20 b's for any b, as input to...

16 August 2020 5,036 3 View

Is it possible to excite P3HT film around 800 nm in PL?

I had prepared the P3Ht film (~100nm) and recorded the PL spectra at an excitation wavelength of 780 nm and the emission spectra resembles the similarity to that recorded at excitation wavelength...

17 July 2019 9,422 2 View

When not as important to consider solvent effects?

We consider solvent effects to be important for polar molecules, But why are they not important for non-polar/hydrophobic molecules?

14 April 2018 9,931 4 View

When is it necessary to consider size consistency?

I understand that if a given theory for the evaluation of the energy is size consistent, then the energy of the supersystem A+B, separated by a sufficiently large distance so there is essentially...

25 March 2018 5,908 2 View

Any expereince about gluteraldehyde crosslinking?

I am trying to crosslink my protein by gluteraldehyde but after crosslinking multimeric form of protein is not able to run on SDS PAGE even after 6 hrs long run at 90V. Please share your experience.

20 March 2018 665 3 View

What is the suitability of PM6, HF, CCSD(T) and B3LYP for these reactions?

To model the first bond dissociation energy of a reaction like: MX6 --> MX5 + X I understand HF is not a suitable level of theory as it cannot describe bond dissociation due to its inability to...

17 March 2018 7,087 4 View

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27 July 2017 1,748 3 View

Problem with PCR amplification ?

I am trying to amplify a gene in same conditions (i.e. melting temp, annealing temp and extension temp and time for all steps) on which I have previously amplified this gene. But this time I am...

21 January 2017 7,442 3 View

Rosetta cells in autoinduction media?

Hello I have cultured rosetta cells in ZYP5052 autoinduction media. It is showing very slow growth. Does anyone have some experience with this media? please share .

13 December 2016 9,518 3 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View