What is the best method of preparing cell supernatant samples for a Luminex assay to detect cytokines ?

I am planning to run a Luminex assay using cell supernatant to assess cytokine secretion by tumor cells. I came across different methods in literature; some suggest seeding in complete media and...

03 February 2021 4,867 3 View

Which staining method is best for Archaea?

Generally to differentiate between bacteria we use gram staining technique, broadly based on the composition of their cell wall. However archaebacteria have different cell wall composition, which...

14 December 2020 9,703 5 View

How do I dissolve or disperse ceramic compound Lithium zirconium phosphate in NMP or any other organic solvent?

I want to form composite of pvdf with ceramic compound to dissolve pvdf I am using NMP, how will I dissolve ceramic.

21 August 2018 978 3 View

When not as important to consider solvent effects?

We consider solvent effects to be important for polar molecules, But why are they not important for non-polar/hydrophobic molecules?

14 April 2018 9,931 4 View

When is it necessary to consider size consistency?

I understand that if a given theory for the evaluation of the energy is size consistent, then the energy of the supersystem A+B, separated by a sufficiently large distance so there is essentially...

25 March 2018 5,908 2 View

What is the suitability of PM6, HF, CCSD(T) and B3LYP for these reactions?

To model the first bond dissociation energy of a reaction like: MX6 --> MX5 + X I understand HF is not a suitable level of theory as it cannot describe bond dissociation due to its inability to...

17 March 2018 7,087 4 View

What is the maximum SLS concentration that can be used in enteric coatings?

13 November 2015 1,055 3 View

Any protocol for Biochemical test for fungi and algae?

For the bacterial identification we also go for biochemical profiling, similarly if we are going to identify a novel fungi, can we perform some biochemical test?

01 January 1970 1,126 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View