I'm trying to dilute hematoxylin (I'm using Surgipath Selectech). I'm not sure what I should use to dilute the solution.
I am trying to get a very light stain on the nuclei of cryosections of mouse embryos. We've shortened the time in the hematoxylin to a quick dip and it is still too dark. So if anyone has any ideas beyond dilution I would greatly appreciate it!
Thank you!
Good day, dear collegue.
What kind of hematoxylin stain you need? Hematoxylin by Mayer, by Harris, by Mallory, by Erlich or Ganzen?
Hi Tanya, I think you might also want to look at at a previous thread in ResearchGate I post below.
Anyway if you want to dilute what you have, I would do it in 5 mM citric acid (1:2 -1:4 dilution), which is what you use to prepare it (see second link). The acid dilution should reduce it's staining efficiency.
https://www.researchgate.net/post/Can_we_dilute_Hematoxylin_prior_using_for_IHC
http://cshprotocols.cshlp.org/content/2008/7/pdb.rec11389.full?text_only=true
I often dilute a city water (1:10 dilution). Then kept in the dark at least one night to remove some precipitate. Carefully collect the diluted solution without mixing the bottom. The diluted solution never overstain. This diluted solution can be possible to use at least 1-2 weeks.
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