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Questions related from Tanya Casta
Hi all! Our lab is using the vector elite ABC kit for IHC in brain tissue. We go through a ton of these reagents, and as we all well know, they are expensive. I'm wondering if anyone has...
02 May 2018 820 1 View
Hi all! Our lab is trying to get all of our rules and protocols straightened out and one that we aren't sure about is the best way to store and take out cryosectioned slides. We currently add...
17 February 2017 929 2 View
Hi all! I am currently working on a project where we have gone from using mouse embryos (E7.5-9.0) to pig embryos (E.17-20). We have notices of late a lot of issues with the technical aspect of...
15 December 2016 2,856 4 View
We are working on staining adult mouse intestines. They are cryo sections. We are getting non-specific binding from the secondary antibodies when no primaries are present. This is supposed to...
26 October 2016 9,314 9 View
Our current protocol has us fixing slides (cryo or paraffin) for 24 hours. It was just brought to my attention that this is wrong, but I was not told how long is correct. I would love to know...
03 August 2015 3,919 11 View
I'm trying to dilute hematoxylin (I'm using Surgipath Selectech). I'm not sure what I should use to dilute the solution. I am trying to get a very light stain on the nuclei of cryosections of...
09 June 2015 8,446 4 View
I'm having a lot of trouble sectioning nerve tissue (sciatic and ovarian). I can get any decent longitudinal cuts, the tissue catches and shreds or completely disintegrates in the water bath. I...
19 May 2015 1,594 3 View
I am wondering if anyone can help me. I have some cadaver skin tissues that were processed, however I did not get proper infiltration. I used an extended human process (etoh 70 - 2 hr, etoh 80 -...
26 August 2014 7,760 8 View
