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Questions related from Tanya Casta
Hi all! Our lab is using the vector elite ABC kit for IHC in brain tissue. We go through a ton of these reagents, and as we all well know, they are expensive. I'm wondering if anyone has...
02 May 2018 759 1 View
Hi all! I am currently working on a project where we have gone from using mouse embryos (E7.5-9.0) to pig embryos (E.17-20). We have notices of late a lot of issues with the technical aspect of...
15 December 2016 2,778 4 View
We are working on staining adult mouse intestines. They are cryo sections. We are getting non-specific binding from the secondary antibodies when no primaries are present. This is supposed to...
26 October 2016 9,250 9 View
Our current protocol has us fixing slides (cryo or paraffin) for 24 hours. It was just brought to my attention that this is wrong, but I was not told how long is correct. I would love to know...
03 August 2015 3,851 11 View
I'm trying to dilute hematoxylin (I'm using Surgipath Selectech). I'm not sure what I should use to dilute the solution. I am trying to get a very light stain on the nuclei of cryosections of...
09 June 2015 8,399 4 View
I am wondering if anyone can help me. I have some cadaver skin tissues that were processed, however I did not get proper infiltration. I used an extended human process (etoh 70 - 2 hr, etoh 80 -...
26 August 2014 7,686 8 View
