I have to design primers for different isoforms of leptin receptor gene.
Can anyone plese tell me how I can design them so they are specific for one isoform.
Thanks
Surabhi
It dependents on the difference between the isoforms, single amino acids or splice variants? In case of single amino acids its like the analysis of SNPs, you need common primers to amplify the region and allele specific probes. In case of splice variants you can choose primer pairs which amplify only specific exon combinations.
It dependents on the difference between the isoforms, single amino acids or splice variants? In case of single amino acids its like the analysis of SNPs, you need common primers to amplify the region and allele specific probes. In case of splice variants you can choose primer pairs which amplify only specific exon combinations.
Hi Surabhi,
See there cDNA sequences, align them in a program and look for the region of differences to design the isoform specifc primers....then you must check the specificity of the designed primers by programs such as PrimerBLAST at NCBI website..
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