I am planning to microinject a drug into the lateral (LA) and central amygdala (CeA) of male wistar rats and observe their behavior. Following that, I want to conduct a western blot and RT-PCR analysis. I realise that I have to validate the microperfusion in the LA and CeA through staining of the brain. So, how do I extract the LA and CeA nuclei from these experimental animals for WB and RT-PCR analysis? Do I need a separate group of rats? The problem is the drug can’t be administered via systemic injection as it doesn’t pass through the blood brain barrier.
Dear Dr Laura,
Thank you for the reply.
Regarding the administration of the drug in the lateral ventricle, is there a standard period of time that I need to wait prior to conducting the behavioral study?
How do I ensure the drug binding in the LA and CeA as I will be assessing the behaviors specific to amygdala function?
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