How do I circularize a TOPO vector?

09 November 2022 0 5K Report

I am trying to figure out how to make more TOPO vector. I'm using the PCR4Blunt-TOPO vector which is already linearized. In the kit there are only 25 ul of 10 ng/ul TOPO vector and I have do to several transformations using this vector. I was therefore wondering if it is possible to make more TOPO vector by doing transformation just without adding any insert. So basically I want the TOPO vector to self-ligate. The vector has blunt ends and as far as I understand, there are phosphates missing on the ends of the backbone, making it harder to self-circularize. Any suggestions on how this could be done?

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