30 October 2023 2 3K Report

One day my HPLC peaks were nice and tall, narrow, almost perfectly Gaussian, and the next day they were short and fat.

Same sample in the same solvent

Same HPLC method

Measured the mobile phase flow rate and it was correct

So I call the column manufacturer and they suggested:

Smaller sample loop - still short and fat

Lower concentration - still short and fat

Completely different column (C-18) - still short and fat.

Any ideas?

Thank you

Kevin

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