One day my HPLC peaks were nice and tall, narrow, almost perfectly Gaussian, and the next day they were short and fat.
Same sample in the same solvent
Same HPLC method
Measured the mobile phase flow rate and it was correct
So I call the column manufacturer and they suggested:
Smaller sample loop - still short and fat
Lower concentration - still short and fat
Completely different column (C-18) - still short and fat.
Any ideas?
Thank you
Kevin