Selma Tausen Olsen

2 Questions 1 Answers 0 Followers

Questions related from Selma Tausen Olsen

How do I circularize a TOPO vector?

I am trying to figure out how to make more TOPO vector. I'm using the PCR4Blunt-TOPO vector which is already linearized. In the kit there are only 25 ul of 10 ng/ul TOPO vector and I have do to...

09 November 2022 4,535 0 View

How do i self-ligate a linearized vector without insert and do transformation to increase the amount of vector?

Hi everyone. I am working on making RNA probes for in situ hybridization, and am at the moment about to transform some competent cells. However, I just realized that the concentration of the...

11 October 2022 5,640 1 View