Does cleaning up cDNA after reverse transcription reduce late cycle background?

I've been wondering if removing the random primers from the reverse transcription reaction either with a column or precipitation before using it as a qPCR template reduces the background a bit in...

25 December 2020 6,258 4 View

How to find porosity of a sintered ceramic compact ?

I want to know the porosity of my sintered ceramic composite ? Please help me out

21 December 2020 4,343 6 View

Can I include multiple outcome measures of the same construct, from the same study, in a meta-analysis?

I am carrying out a meta-analysis that looks at cognitive inhibition as an outcome. A wide range of assessment types can be used for this outcome and sometimes a study will include two or three...

15 November 2020 7,055 1 View

Can anyone suggest me an industry working in Biostimulants ?

it would be very helpful if anyone would suggest industries working in biostimulants.

25 October 2020 5,824 2 View

How can we confirm the formation of solid solution by using EDX point analysis and elemental mapping ?

I fabricated a composite say AB and both are carbides ceramics having formula AC and BC. I did EDX point analysis and observed A (45 wt %) and B (28 wt %), Carbon (26 wt %) and Oxygen (1 wt %)....

20 October 2020 7,419 7 View

Docking Approach for Combinational Drugs?

Docking studies are simple when it is a one ligand-one protein approach. However, what is the protocol for studying the combinational effect of 2 drug molecules on a particular protein, where we...

02 October 2020 8,009 22 View

How do you use the span and wagner equation of state (EOS) to calculate the mass of CO2 in a given volume of cylinder?

I am trying to use the equation of state of pure CO2 to calculate the required mass of co2 that could be contained in a given volume of cylinder.

24 September 2020 5,581 2 View

Pooling multiple continuous variables from the same study?

I am looking to meta-analyze the differential effects of physical abuse and neglect on depression in a meta-analysis. If I come across a study the reports a correlation between a continuous...

24 September 2020 4,211 3 View

How to calculate sample size for 3 way Mixed Between Within Subjects ANOVA?

Hi all, I have been trying to figure out a way to calculate a priori sample size for a mixed anova. I have a study that uses 2 x 7 x 2. The last IV is within subjects. I appreciate any help....

23 September 2020 3,635 3 View

Should I subtract a blank if the measurement for the blank is below the limit of detection?

I am working with metabolite data from an experiment that looked at the exometabolome in filtrate of bacteria grown in a medium made with natural seawater. Because natural seawater is a complex...

15 June 2020 385 11 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View