How Do I Analyse Microscopic images of JC-1 staining for MMP?

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Gopal Agarwal

For microscopic image, i guess you can use image J software developed by NIH.

After counting the JC-1 green positive mitos and JC1 postive red mitos, you can easily count their ratio.

Hope it is useful to you.

Patel Bharat

You can total count and analyze total area by ImageJ. For Area calculation go through color threshold.