Sourav Kumar

4 Questions 4 Answers 0 Followers

Questions related from Sourav Kumar

I have found same signal from control and sample in DOT-BLOT Assay. What is the precaution needed for optimization of this?

I am trying to Generate polyclonal antibodies towards the amyloid-like species of glucosylcerebrocide (GlcCer). Rabbit antisera were collected and used against this GlcCer. I have prepared GlcCer...

13 March 2022 4,654 3 View

How Do I Analyse Microscopic images of JC-1 staining for MMP?

How do I calculate Jc-1 monomer/JC-1 aggregates ratio from microscopic images?

07 May 2021 404 2 View

I am finding a lot of cellular debris in the culture flask? What are the reason behind it?

I am facing a problem that cellular debris observed in my SHSY5Y cells. I have seeded the cells in DMEM media with 10% FBS in T-25/ 60mm dish (antibiotic used PEN-Strepand gentamycin). I have also...

18 January 2021 3,423 3 View

Same Signal coming from control and sample well.

I am trying to Generate polyclonal antibodies towards the amyloid-like species of glucosylcerebrocide (GlcCer). Rabbit antisera were collected and used against this GlcCer. I have prepared GlcCer...

01 January 1970 6,525 2 View