My department recently got a hold of an ECIS-Z machine for measuring cell monolayer impedance in real-time. I have been having issues getting the 96-well plate to properly align with the 96W array station. I always have between 2-6 wells fail the initial SETUP when the device is checking connectivity (occurs randomly). The department that loaned us the device did provide the 96-well plates and I noticed that they are 3 years old. My thinking is that the plates themselves are bad due to being so far out of date. I was wondering how difficult the procedure is with brand new plates when you initially insert them into the 96W array station. I have ordered some brand new plates so I hope I have better success once they arrive. Any advice or experience using the device is greatly appreciated.
Chris Delavan Mounting of the 96well ECIS plate to the reader should not be difficult at all. All you need to do is to use one hand to firmly press the plate onto the reader while shifting the left latch to the right. Then continue to press your plate and shift the right latch to the left. You should feel that your plate sits firmly on the reader. I don't think that expired plates will give you issues with connectivity unless they were kept open and electrodes are corroded. What type of 96 well plate do you use? 20, 10 or 1 electrode?
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