Chris Delavan

6 Questions 5 Answers 0 Followers

Questions related from Chris Delavan

ExoQuick-TC exosome isolation kit and pellet formation?

I am attempting to harvest exosomes from both human bone marrow and adipose MSCs using the ExoQuick-TC kit from System Biosciences. I followed the protocol from the company but after incubation...

07 April 2023 925 1 View

Enzymatic Digestion for Endothelial Cell Isolation?

I was wondering if anybody has an optimal enzyme concentration for Collagenase, Dispase, and DNaseI for the tissue digestion of rat small intestine to obtain a good cell suspension for MACS CD31+...

19 October 2021 2,655 2 View

How difficult to mount the 96-well array for Electric Cell-Substrate Impedance Sensing (ECIS)?

My department recently got a hold of an ECIS-Z machine for measuring cell monolayer impedance in real-time. I have been having issues getting the 96-well plate to properly align with the 96W...

24 June 2019 4,201 1 View

Transfection of human mesenchymal stem cells?

I was wondering if anyone has tried transfecting human MSCs with an shRNA plasmid using the HUSH brand of plasmids from Origene along with their transfection reagent turbofectin 8.0. I am having...

07 March 2019 6,026 5 View

Has anyone encountered this problem with a BBB transwell permeability assay?

I am currently attempting to establish an assay to evaluate BBB permeability with hCMEC/D3 cells and I am running into an issue with attempting to disrupt the TJ proteins to cause leakage. I am...

01 February 2019 5,509 4 View

Best way to change out media from Transwell inserts?

I was curious as to what technique other people use to change out media from the inner chamber of Corning transwell inserts. It can be a bit of a challenge not to disrupt the cellular monolayer...

19 April 2018 9,831 3 View