How diagnostic CD4 absolute count is done by flow cytometry without trucount beads?

08 December 2022 1 8K Report

Can anyone please help me in calculating flow cytometry absolute CD4 count without using trucount beads.

Is it really possible without beads?

Only if you have a flow cytometer equipped with a syringe like system that allows absolute counts. A couple of flow cytometers are equipped or can be equipped with such a tool (i.e. MacsQuant (Miltenyi), Quanteon (Agilent), FacsVerse (BD).

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