If we store the sample at -20 degrees and remove it for cyanotoxin analysis after 2 days, what's the minimum time we need to defrost the sample to get stable results from LC-MS? Also after defrosting the samples we have noticed air bubbles inside the vials. Would that have a great impact on the results of cyanotoxin analysis using LC-MS? If yes then is there any decent way to remove these air bubbles?
If you do not have data availability, better to wait until the sample arrives to the room temperature. You may record that time. Usually free thaw results could be available in literature.
During method validation you had have to check stability at RT. Depends on that time, you can remain your samples at RT until complete thawing. Yes, bubble gives false results. So, try to remove bubbles (even you can tap gently) before run at HPLC. Hope you understand.
This will be helpful. Thank you Nayan Chandra Mohanto and Udaya Jayasundara
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