I am working on detection of some proteins secreted from cell associated with cell migrations and I try to notice migration of these cancer cells but they don't migrate. What I should add to these cells to make them to migrate.
Irfan Khan
Hi, are you using Transwell chamber for cell migration assay? try to use DMEM/high glucose medium containing 10% FBS (Fetal Bovine serum)
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Mohammed A. A. Abdullah
No, we use another microchip for qualitative assay. I usually used these media and no migration is observed even after 24 hrs. I notice migration for macrophages only after adding LPS.
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Michael Deci
Hello, you could use a chemokine such as CCL2 or CXCL12 to induce migration via the CCR2 and CXC4 pathway, respectively.
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