You may use any standard protocol for cell homogenisation with a probe sonicator.

This link for a CSH protocol may help

https://www.researchgate.net/publication/223967686_Immunoprecipitation_Lysing_Bacteria_by_Sonication

I work with cell suspensions and I use to sonicate them to break the cell wall in order to extract proteins. I use a probe sonicator, stablishing 10% of potency (the lowest) and I keep sonicating the sample for 10-20 seconds in ice. Then you will have a cell homogenate which is recommended some kind of centrifugation to remove heavy/ non-broken components.

@Ayah, may you elaborate the question, as what analytes you want to measure by ELISA? If you may share catalogue number of kit.

You either need to separate the plasma and sonicate it. 10 sonication cycle may be used ; 10 second pulse and 30 sec rest.

If you want to analyze cellular components the you should lyse the erythrocytes and centrifuge to get your cells in pellete. Now you should sonicate the cells (20kHz for 10sec pulse with 30 sec rest on ice).

Your RBC (even after lysis may interfare with absorption as it has red color).

Further you should centrifuge the sonicated tube at 15000-20000 g for 30 minutes to remove all the debris. Collect the supernatent for ELISA.

Good answer Jitendra Kumar

Jitendra kumar

Thank you so much.

I want to measure an intracellular protein expressed by WBC's

Would you please send me the whole protocol i have one looks like what you said but they didn't mention the buffer name or its concentration.

And Thank you again.

Thank you Biswajoy and Jaun.